A Functional Assay for Putative Mouse and Human Definitive Endoderm using Chick Whole-Embryo Cultures
نویسندگان
چکیده
منابع مشابه
Gene transfer into mouse prepancreatic endoderm by whole embryo electroporation.
CONTEXT Understanding gene function in the developing pancreas is a major issue for pancreatic cell therapy. The in vivo analysis of gene function has essentially been performed by modulating gene expression in transgenesis. A faster and easier method is electroporation of mouse embryos. This technique, coupled with whole embryo culture, enables one to deliver genes and analyze their effects in...
متن کاملstauprimide priming of human embryonic stem cells toward definitive endoderm
objective: in vitro production of a definitive endoderm (de) is an important issue in stem cell-related differentiation studies and it can assist with the production of more efficient endoderm derivatives for therapeutic applications. despite tremendous progress in de differentiation of human embryonic stem cells (hescs), researchers have yet to discover universal, efficient and cost-effective ...
متن کاملSequential allocation and global pattern of movement of the definitive endoderm in the mouse embryo during gastrulation.
During mouse gastrulation, endoderm cells of the dorsal foregut are recruited ahead of the ventral foregut and move to the anterior region of the embryo via different routes. Precursors of the anterior-most part of the foregut and those of the mid- and hind-gut are allocated to the endoderm of the mid-streak-stage embryo, whereas the precursors of the rest of the foregut are recruited at later ...
متن کاملImproved Assay Technique for Coxiella Burnetii in Chick Embryo.
The egg 50% lethal doses (IiD5o) for Coxiella burnetii range from 105 to 10-6, whereas the 50% infectious doses (ID5o) determined by microscopic examination range from 10-10 to 10-11 (Weiss and Pietryk, J. Bacteriol. 72:235, 1956; Pickens and Gaon, Am. J. Trop. Med. Hyg. 10:49, 1961). If the LDno value for eggs could be increased, dilute suspensions of rickettsiae could be assayed without the t...
متن کاملAssay for Neural Induction in the Chick Embryo
The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ideal tool for studying the formation and initial patterning of the nervous system. This video demonstrates how to graft organizer tissue into a host, a method by which Hensen s node (the organizer in the chick embryo) is grafted to a host compete...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Stem Cell Research & Therapy
سال: 2012
ISSN: 2157-7633
DOI: 10.4172/2157-7633.1000128